Zheng B., Zhu Y., Sardans J., Peñuelas J., Su J. (2018) QMEC: a tool for high-throughput quantitative assessment of microbial functional potential in C, N, P, and S biogeochemical cycling. Science China Life Sciences. : 0-0.EnlaceDoi: 10.1007/s11427-018-9364-7
Microorganisms are major drivers of elemental cycling in the biosphere. Determining the abundance of microbial functional traits involved in the transformation of nutrients, including carbon (C), nitrogen (N), phosphorus (P) and sulfur (S), is critical for assessing microbial functionality in elemental cycling. We developed a high-throughput quantitative-PCR-based chip, Quantitative microbial element cycling (QMEC), for assessing and quantifying the genetic potential of microbiota to mineralize soil organic matter and to release C, N, P and S. QMEC contains 72 primer pairs targeting 64 microbial functional genes for C, N, P, S and methane metabolism. These primer pairs were characterized by high coverage (average of 18–20 phyla covered per gene) and sufficient specificity (>70% match rate) with a relatively low detection limit (7–102 copies per run). QMEC was successfully applied to soil and sediment samples, identifying significantly different structures, abundances and diversities of the functional genes (P
Fernández-Martínez M., Vicca S., Janssens I.A., Ciais P., Obersteiner M., Bartrons M., Sardans J., Verger A., Canadell J.G., Chevallier F., Wang X., Bernhofer C., Curtis P.S., Gianelle D., Grünwald T., Heinesch B., Ibrom A., Knohl A., Laurila T., Law B.E., Limousin J.M., Longdoz B., Loustau D., Mammarella I., Matteucci G., Monson R.K., Montagnani L., Moors E.J., Munger J.W., Papale D., Piao S.L., Peñuelas J. (2017) Atmospheric deposition, CO2, and change in the land carbon sink. Scientific Reports. 7: 0-0.EnlaceDoi: 10.1038/s41598-017-08755-8
Concentrations of atmospheric carbon dioxide (CO2) have continued to increase whereas atmospheric deposition of sulphur and nitrogen has declined in Europe and the USA during recent decades. Using time series of flux observations from 23 forests distributed throughout Europe and the USA, and generalised mixed models, we found that forest-level net ecosystem production and gross primary production have increased by 1% annually from 1995 to 2011. Statistical models indicated that increasing atmospheric CO2 was the most important factor driving the increasing strength of carbon sinks in these forests. We also found that the reduction of sulphur deposition in Europe and the USA lead to higher recovery in ecosystem respiration than in gross primary production, thus limiting the increase of carbon sequestration. By contrast, trends in climate and nitrogen deposition did not significantly contribute to changing carbon fluxes during the studied period. Our findings support the hypothesis of a general CO2-fertilization effect on vegetation growth and suggest that, so far unknown, sulphur deposition plays a significant role in the carbon balance of forests in industrialized regions. Our results show the need to include the effects of changing atmospheric composition, beyond CO2, to assess future dynamics of carbon-climate feedbacks not currently considered in earth system/climate modelling. © 2017 The Author(s).
Gargallo-Garriga A., Ayala-Roque M., Sardans J., Bartrons M., Granda V., Sigurdsson B.D., Leblans N.I.W., Oravec M., Urban O., Janssens I.A., Peñuelas J. (2017) Impact of soil warming on the plant metabolome of Icelandic grasslands. Metabolites. 7: 0-0.EnlaceDoi: 10.3390/metabo7030044
Climate change is stronger at high than at temperate and tropical latitudes. The natural geothermal conditions in southern Iceland provide an opportunity to study the impact of warming on plants, because of the geothermal bedrock channels that induce stable gradients of soil temperature. We studied two valleys, one where such gradients have been present for centuries (long-term treatment), and another where new gradients were created in 2008 after a shallow crustal earthquake (short-term treatment). We studied the impact of soil warming (0 to +15° C) on the foliar metabolomes of two common plant species of high northern latitudes: Agrostis capillaris, a monocotyledon grass; and Ranunculus acris, a dicotyledonous herb, and evaluated the dependence of shifts in their metabolomes on the length of the warming treatment. The two species responded differently to warming, depending on the length of exposure. The grass metabolome clearly shifted at the site of long-term warming, but the herb metabolome did not. The main up-regulated compounds at the highest temperatures at the long-term site were saccharides and amino acids, both involved in heat-shock metabolic pathways. Moreover, some secondary metabolites, such as phenolic acids and terpenes, associated with a wide array of stresses, were also up-regulated. Most current climatic models predict an increase in annual average temperature between 2–8° C over land masses in the Arctic towards the end of this century. The metabolomes of A. capillaris and R. acris shifted abruptly and nonlinearly to soil warming >5° C above the control temperature for the coming decades. These results thus suggest that a slight warming increase may not imply substantial changes in plant function, but if the temperature rises more than 5° C, warming may end up triggering metabolic pathways associated with heat stress in some plant species currently dominant in this region. © 2017 by the authors. Licensee MDPI, Basel, Switzerland.
Gargallo-Garriga A., Wright S.J., Sardans J., Pérez-Trujillo M., Oravec M., Večeřová K., Urban O., Fernández-Martónez M., Parella T., Penuelas J. (2017) Long-term fertilization determines different metabolomic profiles and responses in saplings of three rainforest tree species with different adult canopy position. PLoS ONE. 12: 0-0.EnlaceDoi: 10.1371/journal.pone.0177030
Background Tropical rainforests are frequently limited by soil nutrient availability. However, the response of the metabolic phenotypic plasticity of trees to an increase of soil nutrient availabilities is poorly understood. We expected that increases in the ability of a nutrient that limits some plant processes should be detected by corresponding changes in plant metabolome profile related to such processes. Methodology/Principal findings We studied the foliar metabolome of saplings of three abundant tree species in a 15 year field NPK fertilization experiment in a Panamanian rainforest. The largest differences were among species and explained 75% of overall metabolome variation. The saplings of the large canopy species, Tetragastris panamensis, had the lowest concentrations of all identified amino acids and the highest concentrations of most identified secondary compounds. The saplings of the amid canopyo species, Alseis blackiana, had the highest concentrations of amino acids coming from the biosynthesis pathways of glycerate-3P, oxaloacetate and - ketoglutarate, and the saplings of the low canopy species, Heisteria concinna, had the highest concentrations of amino acids coming from the pyruvate synthesis pathways. Conclusions/Significance The changes in metabolome provided strong evidence that different nutrients limit different species in different ways. With increasing P availability, the two canopy species shifted their metabolome towards larger investment in protection mechanisms, whereas with increasing N availability, the sub-canopy species increased its primary metabolism. The results highlighted the proportional distinct use of different nutrients by different species and the resulting different metabolome profiles in this high diversity community are consistent with the ecological niche theory. © 2017 Gargallo-Garriga et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Goll, D.S., Vuichard, N., Maignan, F., Jornet-Puig, A., Sardans, J., Violette, A., Peng, S., Sun, Y., Kvakic, M., Guimberteau, M., Guenet, B., Zaehle, S., Penuelas, J., Janssens, I., Ciais, P. (2017) A representation of the phosphorus cycle for ORCHIDEE (revision 4520). Geoscientific Model Development. 10: 3745-3770.EnlaceDoi: 10.5194/gmd-10-3745-2017
Grau, O., Peñuelas, J., Ferry, B., Freycon, V., Blanc, L., Desprez, M., Baraloto, C., Chave, J., Descroix, L., Dourdain, A., Guitet, S., Janssens, I.A., Sardans, J., Hérault, B. (2017) Nutrient-cycling mechanisms other than the direct absorption from soil may control forest structure and dynamics in poor Amazonian soils. Scientific Reports. 7: 0-0.EnlaceDoi: 10.1038/srep45017
Luo, W., Li, M.-H., Sardans, J., Lü, X.-T., Wang, C., Peñuelas, J., Wang, Z., Han, X.-G., Jiang, Y. (2017) Carbon and nitrogen allocation shifts in plants and soils along aridity and fertility gradients in grasslands of China. Ecology and Evolution. : 0-0.EnlaceDoi: 10.1002/ece3.3245
Margalef O., Sardans J., Fernández-Martínez M., Molowny-Horas R., Janssens I.A., Ciais P., Goll D., Richter A., Obersteiner M., Asensio D., Peñuelas J. (2017) Global patterns of phosphatase activity in natural soils. Scientific Reports. 7: 0-0.EnlaceDoi: 10.1038/s41598-017-01418-8
Soil phosphatase levels strongly control the biotic pathways of phosphorus (P), an essential element for life, which is often limiting in terrestrial ecosystems. We investigated the influence of climatic and soil traits on phosphatase activity in terrestrial systems using metadata analysis from published studies. This is the first analysis of global measurements of phosphatase in natural soils. Our results suggest that organic P (Porg), rather than available P, is the most important P fraction in predicting phosphatase activity. Structural equation modeling using soil total nitrogen (TN), mean annual precipitation, mean annual temperature, thermal amplitude and total soil carbon as most available predictor variables explained up to 50% of the spatial variance in phosphatase activity. In this analysis, Porg could not be tested and among the rest of available variables, TN was the most important factor explaining the observed spatial gradients in phosphatase activity. On the other hand, phosphatase activity was also found to be associated with climatic conditions and soil type across different biomes worldwide. The close association among different predictors like Porg, TN and precipitation suggest that P recycling is driven by a broad scale pattern of ecosystem productivity capacity. © 2017 The Author(s).
Peñuelas, J., Ciais, P., Canadell, J.G., Janssens, I.A., Fernández-Martínez, M., Carnicer, J., Obersteiner, M., Piao, S., Vautard, R., Sardans, J. (2017) Shifting from a fertilization-dominated to a warming-dominated period. Nature Ecology and Evolution. 1: 1438-1445.EnlaceDoi: 10.1038/s41559-017-0274-8
Peñuelas, J., Janssens, I.A., Ciais, P., Obersteiner, M., Krisztin, T., Piao, S., Sardans, J. (2017) Increasing gap in human height between rich and poor countries associated to their different intakes of N and P. Scientific Reports. 7: 0-0.EnlaceDoi: 10.1038/s41598-017-17880-3
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